WASH

• For the handling and washing of oocytes and embryos

• Hepes-bufferes

• All species range

• Warm to 36ºC for 15 minutes before use

•  Used for selection and washing of oocytes

• Volume of Bottles: 25 ml or 50 ml

 

       IVM

• For the in vitro maturation of oocytes

• For cattle, goats, sheep

• Prepare the vials with 400 µl of IVM medium and cover with 250 μl of oil, inject gas, seal and keep refrigerated

• The vials can be shipped up to 3 days before OPU

• INJECT GAS for 5 - 10" in the stock bottle before returning it to the refrigerator Volume of Bottles: 25 ml or 50 ml

 

       IVF

• For the in vitro fertilization of matures oocytes

• Add the H and PHE aliquots and mix

• Prepare the dishes with 70μl of IVF medium and cover with oil (overnight)

• INJECT GAS for 5 - 10" in the stock bottle before returning it to the refrigerator Volume of Bottles: 25 ml or 50 ml

 

       H / PHE

• Supplement to add to the IVF medium

• Add 110 µl of H and 440 µl PHE into 9,450 µl to IVF medium

• Volume of Bottles: 2 ml

 

       IVC

• For the in vitro culture of zygote (day 1) to blastocyst (day 7)

• Prepare the dishes with 100 μl or 500 μl of IVC medium and cover with oil (overnight)

• INJECT GAS for 5 - 10" in the stock bottle before returning it to the refrigerator Volume of Bottles: 25 ml or 50 ml

 

       SPERM

• Percoll dilution medium

• It can be used to wash semen in a gentler centrifugation

• Volume of Bottles: 10 ml or 25 ml

 

       PERCOLL

• Used to prepare the gradient for conventional semen

• Volume of Bottles: 10 ml or 25 ml

 

       PERCOLL SEX

• Used to prepare the gradient for sexed semen

• Volume of Bottles: 10 ml or 25 ml

 

       ET-HEPES

• Buffered medium, with HEPES, to transportation the embryos for up to 12 hours outside the incubator

• Only warm up to 36º C (does not need to add gas)

• To load one embryo in straw, you need around 280 μl of medium (for 50 embryos around 15 ml of medium)

• Embryo transport temperature is between 36°C - 37°C.

• Volume of Bottles: 25 ml or 50 ml

 

       MINERAL OIL

• Sterile oil for overlaying at all stages of in vitro embryo

• Volume of Bottles: 50 ml

 

       MEDIA FOR CRYOPRESERVATION

 

       VITRIFICATION

• The kit consists of 10 ml of each medium, V1 and V2.

• Fraction it into eppendorfs in a volume that you use per day.

• For vitrification of embryos produced in vivo and in vitro.

• In the medium V1, 8 minutes and in the medium V2, 45 sec.

• Keep at temperatures of 25ºC.

• Volume of Bottles: 10 ml

 

       DEVITRIFICATION

• 3-Step Medium Kit - D1, D2 and D3

• To thaw vitrified embryos

• Helps reduce physical damage and better control extracellular fluid osmotic pressure

• Divide it into eppendorfs a volume that you use per day.

• D1 medium - 45 seconds to 1 minute, Temperature 37-38ºC

• D2 medium - 3 minutes.

• Temperature 25ºC D3 medium, 5 minutes.

• Temperature 25ºC

• Volume of Bottles: 10 ml

 

       DT FREEZING

• 3-Step Medium Kit - HOLDING, HDT and EG

• For freezing embryos produced in vivo and in vitro.

• In the holding medium there is no time counting

• HDT medium - 45 sec to 1 minute - room temperature

• EG medium 10 minutes - room temperature.

• Volume of Bottles: 10 ml

• EMBRYO THAWING: straw in the air per 7 seconds and in the water for 30 seconds - temperature 31ºC

• Volume of Bottles: 10 ml