
All courses offer a certificate and teaching material.
IVF COURSE
What you will learn:
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Aspiration of slaughterhouse ovaries and selection of oocytes (morphological classification);
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In vitro maturation of oocytes in plates, microtubes and transport from farm to laboratory;
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In vitro fertilization with frozen semen (Percoll gradient and washing);
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In vitro microdrop culture (feeding) and direct cultivation in 5-well dishes
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Manipulation of zygotes to remove cumulus cells;
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Cleavage and feeding assessment on D3 and D5 (if necessary);
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Embryo classification, bottling and transport of embryos;
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DT Freezing and Vitrification of IVF Embryos;
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List of materials and equipment needed for the IVF laboratory.
OOCYTE SELECTION COURSE
What you will learn:
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Oocyte aspiration from abbitior ovaries
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Rinsing and filtration
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Microscopic evaluation of oocytes (searching, counting and classification)
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Oocyte handling & transport
CRYOPRESERVATION COURSE
What you will learn:
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Vitrification
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Devitrification
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DT freezing
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Thawing