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All courses offer a certificate and teaching material.

 

      IVF COURSE

            What you will learn:   

 

  • Aspiration of slaughterhouse ovaries and selection of oocytes (morphological classification);

  • In vitro maturation of oocytes in plates, microtubes and transport from farm to laboratory;

  • In vitro fertilization with frozen semen (Percoll gradient and washing);

  • In vitro microdrop culture (feeding) and direct cultivation in 5-well dishes

    • Manipulation of zygotes to remove cumulus cells;

    • Cleavage and feeding assessment on D3 and D5 (if necessary);

    • Embryo classification, bottling and transport of embryos;

  • DT Freezing and Vitrification of IVF Embryos;

  • List of materials and equipment needed for the IVF laboratory.

      OOCYTE SELECTION COURSE


      What you will learn:

  • Oocyte aspiration from abbitior ovaries

  • Rinsing and filtration

  • Microscopic evaluation of oocytes (searching, counting and classification)

  • Oocyte handling & transport​

 

       CRYOPRESERVATION COURSE


       What you will learn:

  • Vitrification

  • Devitrification

  • DT freezing

  • Thawing

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